Initially, various raw materials and agricultural side streams will be examined for their suitability as a feedstock in fungal fermentation. During WP1, several analyses will be conducted to determine the nutrient content of the selected feedstocks. Understanding the nutrient composition is essential to estimate the specific nutrient profile each
feedstock provides to the fungi. All data will be shared with other partners to develop various datasets and digital models. Based on the results of this WP, a user editable and public accessible data base of the tested feedstocks will be established.
Considering the operation challenges of modern bioreactors for submerged filamentous fungi cultivations: (1) managing heterogeneity induced by high viscosity, (2) lowering energy requirements for agitation, (3) ensuring high oxygen mass transfer rates and (4) avoiding foaming, cell adhesion and morphology changes throughout the cultivation. The project team will partake in activities aimed at designing and developing individual bioreactor systems, which are adjustable for multiple cultivation process conditions.
The main objective of this WP is to implement data processes, digital models and AI mechanisms for analysis and control of the fermenters/bioreactors, fermentation parameters, fermentation and bioreactor processes and guide decision making for fermentation experiment setups and validation.
Work Package 4 is organized to design the most optimal and efficient downstream process of the output from WP1 and WP2, targeting product applications in WP5. Specific objectives are: (1) developing a suitable downstream process for recovery and purification of fungal biomass targeting the specific application as food ingredients; (2) creating suitable downstream processes for purification of mycoprotein extracted from mycelia targeting the specific application as food and/or feed ingredients; (3) testing, validating, and improving the quality of the mycoprotein; and (4) developing stepwise extraction and/or other cascading strategies for feedstock and fermentation residues.
The main goal of WP5 is to operationalise the product formulation and production for proof-of-concept to demonstrate commercial viability of the mycoproteins, chitin, mannans and other potential residuals obtained from this project. Over this work package, partners will develop food or petfood grade product from the extracted proteins and polysaccharides
such as myco-chitin/chitosans based on the specific product requirements for each application by the end users. Application work will be done on pilot plant equipment ensuring
scalable solutions.
The incorporation of Safety-and-Sustainability-by-Design (SSbD) requirements in the developed processes within ZEST will involve integrating principles that prioritise safety and sustainability throughout the entire extraction process of targeted compounds from food side streams, in order to ensure that the food ingredients are not only safe for
consumption but also have minimal environmental impact throughout their lifecycle. The main goal of WP6 will be to operationalise sets of criteria for SSbD assessment to guide all implicated processes towards human and environmental risk minimisation while maximising the positive impact of food additives.
such as myco-chitin/chitosans based on the specific product requirements for each application by the end users. Application work will be done on pilot plant equipment ensuring
scalable solutions.
WP7's mission is to optimise the project's effect and the transmission of its findings by delivering targeted information to predefined target groups. Some of the actions foreseen are (1) raising awareness among key stakeholders of the project's existence, significance, and innovative practises, procedures, and new products; (2) ensuring that knowledge and activities are properly exchanged among consortium partners; and (3) making use of the networks of the partners and current effective communication tools.such as myco-chitin/chitosans based on the specific product requirements for each application by the end users. Application work will be done on pilot plant equipment ensuring
scalable solutions.
In general terms, WP8 seeks to ensure maximum impact of the ZEST project. More specifically, this work package aims at (1) developing a suitable business model and operational business plan for project outputs and results to maximise the
impact and market uptake of project outcomes in other application areas, (2) testing and validating the business models to sharpen the exploitation strategy, and (3) supporting the exploitation of project results through assessment and mitigation of exploitation risks and proper IPR (Intellectual Property Rights) management.
such as myco-chitin/chitosans based on the specific product requirements for each application by the end users. Application work will be done on pilot plant equipment ensuring
scalable solutions.
WP9 involves coordinating tasks, managing risks, and ensuring that all project elements come together efficiently to deliver the desired outcomes. It encompasses planning, organizing, leading, and controlling resources (such as time, money, people, and technology) to achieve specific goals and objectives within a defined timeline and budget.
such as myco-chitin/chitosans based on the specific product requirements for each application by the end users. Application work will be done on pilot plant equipment ensuring
scalable solutions.